Yeditepe J Dent. 2019; 15(1): 140-145 | DOI: 10.5505/yeditepe.2019.60251

Agresif periodontitisin mikrobiyal ý̇çeriði ve cerrahi olmayan periodontal tedavisi: Ýki olgu sunumu

Dilek Mamaklýoðlu¹, Bahar Eren Kuru², Maribasappa Karched³, Baþak Doðan^4
1Ýstanbul Aydýn Üniversitesi Diþ Hekimliði Fakültesi, Periodontoloji Ana Bilim Dalý, Ýstanbul
²Yeditepe Üniversitesi Diþ Hekimliði Fakültesi, Periodontoloji Ana Bilim Dalý, Ýstanbul
³Kuveyt Üniversitesi, Biyoklinik Bilimler Ana Bilim Dalý, Kuveyt
⁴Marmara Üniversitesi Diþ Hekimliði Fakültesi, Periodontoloji Ana Bilim Dalý, Ýstanbul

Bu olgu raporunun amacý iki generalize agresif periodontitis (GAgP) hastasýnýn subgingival mikrobiyolojik profilini belirlemek ve cerrahi olmayan periodontal tedavinin (COPT) 6 aylýk klinik sonuçlarýný sunmaktýr. Kliniðimize baþvuran iki GAgP hastasýnýn havuzlama yöntemiyle toplanan subgingival örnekleri, 300 tür/filotipin varlýðýný belirlemek için Human Oral Microbiome Identificaition Microarray analizi kullanýlarak incelendi. COPT 3 hafta içinde uygulandý. Klinik parametreler tedavi öncesi, sonrasý 3. ve 6. aylarda kaydedildi. Hastalar, ilk 3 ayda 2 haftada bir, son 3 ayda 4 haftada bir kontrol seanslarýna çaðýrýldý. Tüm örneklerde toplam 61 türe ve iki hastada ortak 32 türe rastlandý. Ýlk hastada 17, diðer hastada 12 farklý tür tespit edildi. Ýki hastada da yüksek seviyede Filifactor alocis, Porpyromonas gingivalis, Campylobacter concisus and rectus, Fusobacterium nucleatum and Desulfobulbus spp. saptanýrken Aggregatibacter actinomycetemcomitans tespit edilmedi. COPT sonrasý altýncý ayda A. actinomycetemcomitans-negatif iki GAgP hastasýnýn tüm klinik parametrelerinde iyileþme olduðu gözlendi. Bilinen periodontal patojenlere ek olarak yüksek seviyedeki Filifactor alocis ve Desulfobulbus spp.’nin varlýðýnýn GAgP ile iliþkilendirilebileceði düþünülmüþtür.

Anahtar Kelimeler: Agresif periodontitis, periodontal tedavi, mikrobiyal genetik, oligonü, kleotid mikroarray

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Microbial composition and non-surgical periodontal treatment of aggressive periodontitis: Two case report

Dilek Mamaklýoðlu¹, Bahar Eren Kuru², Maribasappa Karched³, Baþak Doðan^4
1Istanbul Aydýn University, Faculty of Dentistry, Department of Periodontology, Istanbul, Turkey
²Yeditepe University, Faculty of Dentistry, Department of Periodontology, Istanbul, Turkey
³Kuwait University, Department of Bioclinical Sciences, Kuwait
⁴Marmara University, Faculty of Dentistry, Department of Periodontology, Istanbul, Turke

The objective of this case report was to characterize the subgingival microbiological profiles of two patients with generalized aggressive periodontitis (GAgP) and to evaluate the clinical outcomes of non-surgical periodontal treatment (NSPT) over a 6-months period. Pooled subgingival samples of two patients who referred to our clinic and diagnosed with GAgP were collected and analyzed for the presence of 300 species/phlotypes using Human Oral Microbe Identification Microarray analysis. NSPT was performed within 3-week period. Clinical parameters were measured at baseline, 3 and 6 months after NSPT. Recall visits were performed every 2 weeks during the first 3 months and every 4 weeks up to 6 months. All samples harboured a total of 61 species and 32 species were common in both patients. First patient had 17 and the other had 12 distinct species. High levels of Filifactor alocis, Porpyromonas gingivalis, Campylobacter concisus and rectus, Fusobacterium nucleatum and Desulfobulbus spp. were detected in both patients while Aggregatibacter actinomycetemcomitans was found in none of them. Six months after NSPT, all clinical parameters were improved in two A. actinomycetemcomitans-negative GAgP patients. In addition to well-recognized periodontal pathogens the presence of high levels of Filifactor alocis and Desulfobulbus spp. seem to be associated with GAgP.

Keywords: Aggressive periodontitis, periodontal treatment, microbial genetics, oligonucleotide microarrays

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